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- Dried bean seeds, mechanically disrupted by Ultra-Turrax, were subjected to extraction by the addition (5 ml/g) of 80% methanol aqueous solution containing 0.6% (v/v) of acetic acid. The suspension was stirred for approximately 5 h at 4 °C temperature in darkness, centrifuged for 10 min at 4 °C at 7000 g , and the pellet extracted again at 4 °C overnight as above.
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